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Journal: Memórias do Instituto Oswaldo Cruz
Article Title: Sustained Oropouche virus transmission in Rio de Janeiro’s Atlantic Forest: genomic evidence over a two-year period
doi: 10.1590/0074-02760250181
Figure Lengend Snippet: Oropouche virus (OROV) genomes from Rio de Janeiro State, 2024-2025. (A) Temporal distribution of OROV genomes from Rio de Janeiro (n = 40), aggregated by epidemiological week (represented by bars) for the 2024-2025 surveillance period and concurrently stratified by the administrative region where the infection likely occurred. (B) Cumulative spatial distribution map displaying the locations of sampled OROV genomes at the municipal level throughout the surveillance period. The diameter of the circles represents the total number of genomes sequenced per municipality and their colours reflect the magnitude of the underlying Oropouche epidemic. Both encodings follow the legend on the right of the panel. Areas shaded in green indicate regions of forest coverage. (C) Genotyping of OROV L, M, and S genome segments via maximum-likelihood (ML) phylogenetic trees. Sequences were coloured according to their lineage/clade as indicated in the key located in the upper right corner. Sequences from Rio de Janeiro are indicated in blue and prototypical sequences (OROV, Perdões virus, Madre de Dios virus, and Iquitos virus) in grey. Branch support values, calculated using the approximate Likelihood Ratio Test (aLRT), are annotated on key nodes defining the main lineages. (D) ML phylogenetic tree of concatenated OROV segments. The dataset includes representatives of all clades previously identified within the OROVBR-2019-2024 lineage. Sub-clades containing genomes from Rio de Janeiro are highlighted and annotated with their statistical support values.
Article Snippet: Branch support was assessed using the approximate
Techniques: Virus, Infection